| Conformation Disturbance of CopC by α-Naphthylamine |
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| 作者单位： | Institute of Molecular Science, Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan 030006, P. R. China |
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| 基金项目： | Supported by the National Natural Science Foundation of China(No. 20771068); the Natural Science Foundation of Shanxi Province, China(No. 2007011024) |
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| 摘 要： | It is known that urea and guanidine hydrochloride(GuHCl) induce conformational change of proteins in a certain range of molar ratios. In our research,α-naphthylamine(NA) above 10-4 mol/L at pH 7.0 was discovered to perturb the conformation of CopC,a copper resistant protein with a Greek β-barrel motif; this was reflected by the greater fluorescence quenching and red-shifted emission peak of CopC. The conformation change of CopC was also verified in acrylamide collision experiment by comparing quenching leve...
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| 关 键 词： | 酞菁钴 萘胺 盐酸胍变性 干扰 构象变化 荧光猝灭 丙烯酰胺 碰撞实验 |
Conformation Disturbance of CopC by α-Naphthylamine |
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| LI Hui-qing,ZHENG Xiao-yan,ZHAO Ya-qin , YANG Bin-sheng Institute of Molecular Science,Key Laboratory of Chemical Biology , Molecular Engineering of Ministry of Education,Shanxi University,Taiyuan ,P. R. China. Conformation Disturbance of CopC by α-Naphthylamine[J]. Chemical Research in Chinese University, 2009, 25(3) |
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| Authors: | LI Hui-qing ZHENG Xiao-yan ZHAO Ya-qin YANG Bin-sheng Institute of Molecular Science Key Laboratory of Chemical Biology Molecular Engineering of Ministry of Education Shanxi University Taiyuan P. R. China |
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| Affiliation: | Institute of Molecular Science, Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan 030006, P. R. China |
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| Abstract: | |
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| Keywords: | CopC Conformational change |
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