水溶性铁硫簇纳米粒子的制备及表征

铁硫簇是普遍存在于生物体内的最古老的生命物质之一[1,2]。1960年人们对固氮细菌、亚线粒体片段及哺乳动物的起源进行研究时发现了一种具有高效氧化还原能力的蛋白质,后来被证明是铁硫蛋白,此后对铁硫簇的研究才得以迅速展开。铁硫蛋白结构中包含有铁与巯基丙氨酸中的硫结合成的具有一定构型的铁硫簇合物,基本结构单元主要以Fe2S2、Fe3S4、Fe4S4这3种簇合物的形式而存在。铁硫簇的一个重要结构特征,就是铁原子由无机硫原子以桥的方式联接,同时,蛋白质肽链中半胱胺酸的巯基作为配体与铁原子相结合。这些铁原子以高自旋Fe!或Fe"形式存在…

Preparation and Characterization of Water-soluble Iron-sulfur Cluster Nanoparticles

Water-solube iron-sulfur cluster nanoparticles were synthesized by using tri-sodium citrate as a stabilizer. The effect of factors such as the amount of reactants, the type of reactants, pH value, dripping order was studied on the formation of iron-sulfur cluster nanoparticles. The structure, photo- and electro- chemical performance were characterized by UV-Vis, Raman, TEM, ED, EDS and Cyclic Voltammetry were used to characterize the structure and properties. The UV-Vis peaks at 436, 532 and 636 nm are attributed to the characteristic peaks of [4Fe-4S]⁺ and [2Fe-2S]²⁺. The Raman results indicate that citrate anions have been adsorbed on the surface of iron-sulfur cluster nanoparticles. TEM image shows the particles are about 5 nm, and EDS result further proves that iron-sulfur cluster coated by tri-sodium citrate has been obtained. Cyclic Voltammetry result demonstrates that iron-sulfur cluster has similar electrochemical property to that in organism.