Production of Lipids Containing High Levels of Docosahexaenoic Acid by a Newly Isolated Microalga, <Emphasis Type="Italic">Aurantiochytrium</Emphasis> sp. KRS101 |
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Authors: | Won-Kyung Hong Dina Rairakhwada Pil-Soo Seo Sung-Yong Park Byung-Ki Hur Chul Ho Kim Jeong-Woo Seo |
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Institution: | (1) Microbe-based Fusion Technology Research Center, Jeonbuk Branch Institute, KRIBB, Jeongeup, Jeonbuk, 580–185, South Korea;(2) Department of Biological Engineering, Inha University, Incheon, 402–751, South Korea; |
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Abstract: | In the present study, a novel oleaginous Thraustochytrid containing a high content of docosahexaenoic acid (DHA) was isolated
from a mangrove ecosystem in Malaysia. The strain identified as an Aurantiochytrium sp. by 18S rRNA sequencing and named KRS101 used various carbon and nitrogen sources, indicating metabolic versatility. Optimal
culture conditions, thus maximizing cell growth, and high levels of lipid and DHA production, were attained using glucose
(60 g l−1) as carbon source, corn steep solid (10 g l−1) as nitrogen source, and sea salt (15 g l−1). The highest biomass, lipid, and DHA production of KRS101 upon fed-batch fermentation were 50.2 g l−1 (16.7 g l−1 day−1), 21.8 g l−1 (44% DCW), and 8.8 g l−1 (40% TFA), respectively. Similar values were obtained when a cheap substrate like molasses, rather than glucose, was used
as the carbon source (DCW of 52.44 g l−1, lipid and DHA levels of 20.2 and 8.83 g l−1, respectively), indicating that production of microbial oils containing high levels of DHA can be produced economically when
the novel strain is used. |
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