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Precise Epitope Organization with Self-adjuvant Framework Nucleic Acid for Efficient COVID-19 Peptide Vaccine Construction |
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| Authors: | Dr Fengyun Shen Zijian Xiong Dr Yumin Wu Prof?Dr Rui Peng Yue Wang Dr Lele Sun Prof?Dr Chunhai Fan Prof?Dr Zhuang Liu |
| Institution: | 1. Institute of Functional Nano & Soft Materials (FUNSOM), Jiangsu Key Lab Carbon Based Functional Materials and Devices, Soochow University, 215123 Suzhou, Jiangsu, China
School of Chemistry and Chemical Engineering, Shanghai Jiao Tong University, 201240 Shanghai, China Contribution: Conceptualization (lead), Data curation (lead), Formal analysis (lead), ?Investigation (lead), Methodology (lead), Writing - original draft (lead);2. Institute of Functional Nano & Soft Materials (FUNSOM), Jiangsu Key Lab Carbon Based Functional Materials and Devices, Soochow University, 215123 Suzhou, Jiangsu, China Contribution: Data curation (supporting), ?Investigation (equal);3. Institute of Functional Nano & Soft Materials (FUNSOM), Jiangsu Key Lab Carbon Based Functional Materials and Devices, Soochow University, 215123 Suzhou, Jiangsu, China Contribution: ?Investigation (supporting), Methodology (supporting);4. Institute of Functional Nano & Soft Materials (FUNSOM), Jiangsu Key Lab Carbon Based Functional Materials and Devices, Soochow University, 215123 Suzhou, Jiangsu, China Contribution: Supervision (supporting);5. School of Chemistry and Chemical Engineering, Shanghai Jiao Tong University, 201240 Shanghai, China Contribution: Methodology (supporting);6. School of Life Sciences, Shanghai University, 200444 Shanghai, China;7. School of Chemistry and Chemical Engineering, Shanghai Jiao Tong University, 201240 Shanghai, China Contribution: Supervision (equal), Writing - review & editing (equal);8. Institute of Functional Nano & Soft Materials (FUNSOM), Jiangsu Key Lab Carbon Based Functional Materials and Devices, Soochow University, 215123 Suzhou, Jiangsu, China |
| Abstract: | Peptide vaccines have advantages in easy fabrication and high safety, but their effectiveness is hampered by the poor immunogenicity of the epitopes themselves. Herein, we constructed a series of framework nucleic acids (FNAs) with regulated rigidity and size to precisely organize epitopes in order to reveal the influence of epitope spacing and carrier rigidity on the efficiency of peptide vaccines. We found that assembling epitopes on rigid tetrahedral FNAs (tFNAs) with the appropriate size could efficiently enhance their immunogenicity. Further, by integrating epitopes from SARS-CoV-2 on preferred tFNAs, we constructed a COVID-19 peptide vaccine which could induce high titers of IgG against the receptor binding domain (RBD) of SARS-CoV-2 spike protein and increase the ratio of memory B and T cells in mice. Considering the good biocompatibility of tFNAs, our research provides a new idea for developing efficient peptide vaccines against viruses and possibly other diseases. |
| Keywords: | COVID-19 Epitope Organization Framework Nucleic Acids Peptide Vaccine |