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A Target Recycling Amplification Process for the Digital Detection of Exosomal MicroRNAs through Photonic Resonator Absorption Microscopy |
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| Authors: | Dr Xiaojing Wang Skye Shepherd Dr Nantao Li Dr Congnyu Che Dr Tingjie Song Yanyu Xiong Isabella Rose Palm Dr Bin Zhao Dr Manish Kohli Prof Utkan Demirci Prof Yi Lu Prof Brian T Cunningham |
| Institution: | 1. Nick Holonyak Jr. Micro and Nanotechnology Laboratory, University of Illinois at Urbana-Champaign, Urbana, IL 61801 USA;2. Nick Holonyak Jr. Micro and Nanotechnology Laboratory, University of Illinois at Urbana-Champaign, Urbana, IL 61801 USA
Department of Bioengineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801 USA These authors contributed equally to this work.;3. Nick Holonyak Jr. Micro and Nanotechnology Laboratory, University of Illinois at Urbana-Champaign, Urbana, IL 61801 USA Department of Electrical and Computer Engineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801 USA;4. Nick Holonyak Jr. Micro and Nanotechnology Laboratory, University of Illinois at Urbana-Champaign, Urbana, IL 61801 USA Department of Bioengineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801 USA;5. Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801 USA;6. Nick Holonyak Jr. Micro and Nanotechnology Laboratory, University of Illinois at Urbana-Champaign, Urbana, IL 61801 USA Department of Electrical and Computer Engineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801 USA Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801 USA;7. Nick Holonyak Jr. Micro and Nanotechnology Laboratory, University of Illinois at Urbana-Champaign, Urbana, IL 61801 USA Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801 USA;8. Division of Oncology, University of Utah School of Medicine, Huntsman Cancer Institute, Salt Lake City, 84112 USA;9. Canary Center at Stanford for Cancer Early Detection, Bio Acoustic-MEMS in Medicine (BAMM) Laboratory, Department of Radiology School of Medicine, Stanford University, Palo Alto, CA 94304 USA |
| Abstract: | Exosomal microRNAs (miRNAs) have considerable potential as pivotal biomarkers to monitor cancer development, dis-ease progression, treatment effects and prognosis. Here, we report an efficient target recycling amplification process (TRAP) for the digital detection of miRNAs using photonic resonator absorption microscopy. We achieve multiplex digital detection with sub-attomolar sensitivity in 20 minutes, robust selectivity for single nucleotide variants, and a broad dynamic range from 1 aM to 1 pM. Compared with traditional qRT-PCR, TRAP showed similar accuracy in profiling exosomal miRNAs derived from cancer cells, but also exhibited at least 31-fold and 61-fold enhancement in the limits of miRNA-375 and miRNA-21 detection, respectively. The TRAP approach is ideal for exosomal or circulating miRNA biomarker quantification, where the miRNAs are present in low concentrations or sample volume, with potentials for frequent, low-cost, and minimally invasive point-of-care testing. |
| Keywords: | Amplification Cancer Diagnostics DNA Toehold Probe Exosomal miRNA Photonic Sensor |