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In Situ Self-Assembly of Fluorogenic RNA Nanozipper Enables Real-Time Imaging of Single Viral mRNA Translation |
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| Authors: | Yu-Peng Zhang Prof Zhi-Gang Wang Yi-Fan Tian Lin-Han Jiang Liang Zhao Prof De-Ming Kong Prof Xing Li Prof Dai-Wen Pang Prof Shu-Lin Liu |
| Institution: | 1. State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Biosensing and Molecular Recognition, Research Center for Analytical Sciences, College of Chemistry, and School of Medicine, Nankai University, Tianjin, 300071 P. R. China
These authors contributed equally to this work. Contribution: Conceptualization (equal), Data curation (lead), Formal analysis (equal), Investigation (lead), Methodology (lead), Writing - original draft (equal), Writing - review & editing (equal);2. State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Biosensing and Molecular Recognition, Research Center for Analytical Sciences, College of Chemistry, and School of Medicine, Nankai University, Tianjin, 300071 P. R. China;3. State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Biosensing and Molecular Recognition, Research Center for Analytical Sciences, College of Chemistry, and School of Medicine, Nankai University, Tianjin, 300071 P. R. China Contribution: Data curation (supporting), Formal analysis (equal), Investigation (supporting);4. State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Biosensing and Molecular Recognition, Research Center for Analytical Sciences, College of Chemistry, and School of Medicine, Nankai University, Tianjin, 300071 P. R. China Contribution: Data curation (supporting), Formal analysis (supporting);5. State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Biosensing and Molecular Recognition, Research Center for Analytical Sciences, College of Chemistry, and School of Medicine, Nankai University, Tianjin, 300071 P. R. China Contribution: Investigation (supporting);6. Beijing Institutes of Life Science, Chinese Academy of Science, Beijing, 100101 China |
| Abstract: | Real-time visualization of individual viral mRNA translation activities in live cells is essential to obtain critical details of viral mRNA dynamics and to detect its transient responses to environmental stress. Fluorogenic RNA aptamers are powerful tools for real-time imaging of mRNA in live cells, but monitoring the translation activity of individual mRNAs remains a challenge due to their intrinsic photophysical properties. Here, we develop a genetically encoded turn-on 3,5-difluoro-4-hydroxybenzylidene imidazolinone (DFHBI)-binding RNA nanozipper with superior brightness and high photostability by in situ self-assembly of multiple nanozippers along single mRNAs. The nanozipper enables real-time imaging of the mobility and dynamic translation of individual viral mRNAs in live cells, providing information on the spatial dynamics and translational elongation rate of viral mRNAs. |
| Keywords: | Aptamers Nanozippers Single mRNA Imaging Translation |