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An Enzyme-Loaded Metal–Organic Framework-Assisted Microfluidic Platform Enables Single-Cell Metabolite Analysis |
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| Authors: | Dr Yanfeng Gao Dr Yanping Wang Prof Bangshun He Yongchun Pan Dongtao Zhou Mengqiu Xiong Prof Yujun Song |
| Institution: | 1. College of Engineering and Applied Sciences, State Key Laboratory of Analytical Chemistry for Life Science, Nanjing University, Nanjing, 210023 China
These authors contributed equally to this work.;2. Department of Laboratory Medicine, Nanjing First Hospital, Nanjing Medical University, Nanjing, 210006 China These authors contributed equally to this work.;3. College of Engineering and Applied Sciences, State Key Laboratory of Analytical Chemistry for Life Science, Nanjing University, Nanjing, 210023 China;4. Department of Laboratory Medicine, Nanjing First Hospital, Nanjing Medical University, Nanjing, 210006 China |
| Abstract: | Colonization of cancer cells at secondary sites, a decisive step in tumor metastasis, is strongly dependent on the formation of metastatic microenvironments regulated by intrinsic single-cell metabolism traits. Herein, we report a single-cell microfluidic platform for high-throughput dynamic monitoring of tumor cell metabolites to evaluate tumor malignancy. This microfluidic device empowers efficient isolation of single cells (>99 %) in a squashed state similar to tumor extravasation, and employs enzyme-packaged metal–organic frameworks to catalyze tumor cell metabolites for visualization. The microfluidic evaluation was confirmed by in vivo assays, suggesting that the platform allowed predicting the tumorigenicity of captured tumor cells and screening metabolic inhibitors as anti-metastatic drugs. Furthermore, the platform efficiently detected various aggressive cancer cells in unprocessed whole blood samples with high sensitivity, showing potential for clinical application. |
| Keywords: | Cellular Metabolism Enzyme Catalysis High-Throughput Screening Microfluidics Single-Cell Analysis |