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Systematic Enzymatic Synthesis of dTDP-Activated Sugar Nucleotides |
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| Authors: | Fangyu Wei Rui Yuan Qin Wen Prof Liuqing Wen |
| Institution: | 1. Carbohydrate-Based Drug Research Center, Shanghai Institute of Materia Media, Chinese Academy of Sciences, Shanghai, 201203 China
University of Chinese Academy of Sciences, Beijing, 100049 China These authors contributed equally to this work.;2. School of Chinese Materia Medica, Nanjing University of Chinese Medicine, Nanjing, 210023 China These authors contributed equally to this work.;3. Carbohydrate-Based Drug Research Center, Shanghai Institute of Materia Media, Chinese Academy of Sciences, Shanghai, 201203 China |
| Abstract: | Deoxythymidine diphosphate (dTDP)-activated sugar nucleotides are the most diverse sugar nucleotides in nature. They serve as the glycosylation donors of glycosyltransferases to produce various carbohydrate structures in living organisms. However, most of the dTDP-sugars are difficult to obtain due to synthetic difficulties. The limited availability of dTDP-sugars has hindered progress in investigating the biosynthesis of carbohydrates and exploring new glycosyltransferases in nature. In this study, based on the de novo and salvage biosynthetic pathways, a variety of dTDP-activated sugar nucleotides were successfully prepared in high yields and on a large scale from readily available starting materials. The produced sugar nucleotides could provide effective tools for fundamental research in glycoscience. |
| Keywords: | Biocatalysis Cascade Reactions One-Pot Reactions Sugar Nucleotides |