HPLC Determination of Imidazoles with Variant Anti-Infective Activity in Their Dosage Forms and Human Plasma |
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Authors: | Oday T. Ali Wafaa S. Hassan Ahdab N. Khayyat Ahmad J. Almalki Mahmoud M. Sebaiy |
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Affiliation: | 1.Department of Chemistry, Faculty of Science, Zagazig University, 44519 Zagazig, Egypt;2.Ministry of Education, Baghdad 55509, Iraq;3.Department of Analytical Chemistry, Faculty of Pharmacy, Zagazig University, 44519 Zagazig, Egypt;4.Department of Pharmaceutical Chemistry, Faculty of Pharmacy, King Abdulaziz University, Jeddah 21589, Saudi Arabia; (A.N.K.); (A.J.A.);5.Department of Medicinal Chemistry, Faculty of Pharmacy, Zagazig University, 44519 Zagazig, Egypt |
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Abstract: | A suitable HPLC method has been selected and validated for rapid simultaneous separation and determination of four imidazole anti-infective drugs, secnidazole, omeprazole, albendazole, and fenbendazole, in their final dosage forms, in addition to human plasma within 5 min. The method suitability was derived from the superiority of using the environmentally benign solvent, methanol over acetonitrile as a mobile phase component in respect of safety issues and migration times. Separation of the four anti-infective drugs was performed on a Thermo Scientific® BDS Hypersil C8 column (5 µm, 2.50 × 4.60 mm) using a mobile phase consist of MeOH: 0.025 M KH2PO4 (70:30, v/v) adjusted to pH 3.20 with ortho-phosphoric acid at room temperature. The flow rate was 1.00 mL/min and maximum absorption was measured with UV detector set at 300 nm. Limits of detection were reported to be 0.41, 0.13, 0.18, and 0.15 µg/mL for secnidazole, omeprazole, albendazole, and fenbendazole, respectively, showing a high degree of the method sensitivity. The method of analysis was validated according to Food and Drug Administration (FDA)guidelines for the determination of the drugs, either in their dosage forms with highly precise recoveries, or clinically in human plasma, especially regarding pharmacokinetic and bioequivalence studies. |
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Keywords: | HPLC anti-infective imidazole dosage forms human plasma |
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