原位聚合阴离子交换型固相萃取（SPE）微柱

原位聚合阴离子交换型固相萃取（SPE）微柱;原位聚合;SPE微柱;分离富集

Anion-exchange Solid Phase Extraction Micro-column Prepared by In-situ Polymerization

According to characteristics of in-situ polymerization of monolithic column,the monolithic(porous) polymer which was thermally initiated within a glass micro-column was fabricated and applied to anion-(exchange) solid phase extraction(SPE).The effects of the amounts of the functional monomer,the initiator,the cross-(linker),the porogenic solvent,the non-covalent monomer,and the temperature,the atmosphere,and the polymerization time for the reaction on the SPE micro-column properties were discussed.The proportion of all the components and reaction conditions were optimized respectively.The(porous) structure of the polymer was characterized by means of scanning electron microscopy(SEM).The absorption characteristic of the SPE monolithic micro-column was proved by ultra-violet absorption of NO~-_(2) at(214 nm).The experimental results show that the home-made anion exchange micro SPE column is of good(absorption) effect.Absorption efficiency of the micro SPE column could reach 72.4% with off-line sample treatment,and enrichment efficiency could reach 77.9 times with on-line sample treatment.