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Indirect detection in high-performance liquid chromatography
Affiliation:1. Department of Chemistry Punjabi University, Patiala, India;2. Department of Chemistry G.S.S.D.G.S Khalsa College Patiala, India;1. School of Chemical Sciences, Central University of Gujarat, Gandhinagar 382030, Gujarat, India;2. Centre for Applied Chemistry, Central University of Gujarat, Gandhinagar 382030, Gujarat, India
Abstract:Indirect detection is a technically simple method to follow and quantify compounds without inherent detector response in high-performance liquid chromatography. The underlying principle can be expressed in simple equations. These equations indicate clearly how the detection sensitivity can be optimized and how disturbance effects can be avoided when the mobile phase gives detector response.
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