Suppression of phase and amplitude J(HH) modulations in HSQC experiments |
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| Authors: | Laura Castañar Eduard Sistaré Albert Virgili R. Thomas Williamson Teodor Parella |
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| Affiliation: | 1. Servei de Ressonància Magnètica Nuclear, Facultat de Ciències, Universitat Autònoma de Barcelona, Barcelona, Catalonia, Spain;2. Departament de Química, Facultat de Ciències, Universitat Autònoma de Barcelona, Barcelona, Catalonia, Spain;3. NMR Structure Elucidation, Process and Analytical Chemistry, Merck & Co. Inc., Rahway, NJ, USA |
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| Abstract: | The amplitude and the phase of cross peaks in conventional 2D HSQC experiments are modulated by both proton–proton, J(HH), and proton–carbon, 1J(CH), coupling constants. It is shown by spectral simulation and experimentally that J(HH) interferences are suppressed in a novel perfect‐HSQC pulse scheme that incorporates perfect‐echo INEPT periods. The improved 2D spectra afford pure in‐phase cross peaks with respect to 1J(CH) and J(HH), irrespective of the experiment delay optimization. In addition, peak volumes are not attenuated by the influence of J(HH), rendering practical issues such as phase correction, multiplet analysis, and signal integration more appropriate. Copyright © 2014 John Wiley & Sons, Ltd. |
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| Keywords: | NMR HSQC pure in‐phase signals perfect HSQC J(HH) modulation |
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