Part I. A specific electrode for urea. |
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Authors: | G G Guilbault W Stokbro |
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Affiliation: | Chemistry Department A, Technical University of Denmark, 2800 LyngbyDenmark |
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Abstract: | A specific simple enzyme stirrer electrode is described for the assay of urea in blood serum. The enzyme is placed directly on a magnetic stirrer and held in place with a nylon net. The enzyme stirrer both stirs the solution and effects an enzymatic transformation, permitting the direct assay of a substrate such as urea. Potassium, Na+ , NH4+ and other organic and inorganic species present in blood do not interfere. Linear curves are obtained from 5· 10-2M to 1· 10-4M urea with slopes close to Nernstian, 0.95 pH/decade. Urea in blood was assayed with an accuracy of 1.8% and a precision of 2.0% with immobilized urease in the stirrer. The stirrers were used for 15 weeks and over 500 assays with excellent results. |
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