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| 纳升电喷雾串联质谱法确证阿托西班的一级结构 | |
| 引用本文： | 梁艳,赵庆国,刘炳玉,李萍.纳升电喷雾串联质谱法确证阿托西班的一级结构[J].分析化学,2009,37(4). |
| 作者姓名： | 梁艳 赵庆国 刘炳玉 李萍 |
| 作者单位： | 1. 武警总医院药剂科,北京,100039 2. 解放军302医院药剂科,北京,100853 3. 国家生物医学分析中心,北京,100850 |
| 摘 要： | 利用电喷雾串联质谱对合成八肽阿托西班进行了二硫键还原前后的精确分子量测定和一级结构的确证.首先通过全扫描模式测定了其还原前后的精确分子量,然后选择母离子m/z 498.73(双电荷)通过串联质谱(MS/MS)得到碎片离子,采用Y离子的方法测定了阿托西班的序列并对其的修饰位点进行了确证.本方法具有灵敏度高、速度快、样品无需纯化等特点,在多肽类药物一级结构分析方面具有独特的优势. |
| 关 键 词： | 纳升电喷雾串联质谱 阿托西班 二硫键 修饰位点 |
Characterization of Primary Structure of Atosiban by Nanoelectrospray Tandem Mass Spectrometry |
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| LIANG Yan,ZHAO Qing-Guo,LIU Bing-Yu,LI Ping.Characterization of Primary Structure of Atosiban by Nanoelectrospray Tandem Mass Spectrometry[J].Chinese Journal of Analytical Chemistry,2009,37(4). | |
| Authors: | LIANG Yan ZHAO Qing-Guo LIU Bing-Yu LI Ping |
| Institution: | General Hospital Pharmacy of Armed Police Forces;Beijing 100039;National Center of Biomedical Analysis;Beijing 100850;302 Hospital Pharmacy of People Lliberation Army;Beijing 100039 |
| Abstract: | The molecular weights of unreduced and reduced for the disulfide bond in the atosiban were measured,and the primary structure of atosiban(an artificially synthesized 8AA peptide) was characterized by nanoelectrospray tandem mass spectrometry. Accurate molecular weights were determined firstly by full-scan mass spectrumetry,then fragmentation ions were obtained by selecting parental ions m/z 498.73(double charge) using mass spectrometry(MS/MS),the sequence and modified site of atosiban were confirmed using Y... |
| Keywords: | Nano-electrospray ionization tandem mass spectrometry atosiban disulfide bond modified site |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |