量子点荧光标记在重组噬菌体表面展示肽与胰岛素受体相互作用中的应用

近年来用于生物荧光标记的发光材料越来越多，尤其是具有较高发光效率、巯基羧酸修饰的纳米微粒，如CdSe，CdTe等半导体量子点(Ouantum dots，QDs)在生物荧光标记领域的应用已成为研究热点，QDs标记作为高灵敏度的非同位素荧光分析法，在实时监测生物体内，尤其是细胞内的蛋白质问相互作用以及研究细胞分化、细胞间相互作用方面的优势日益受到人们的重视。用有机荧光染料来标记细胞和生物分子的方法早已有应用嘲，但是传统的荧光染料有较多的缺陷：激发光谱窄；发射光谱很宽；易光漂白和光解。

Application of Quantum Dots Labeling to the Interaction Between Reconstructed-phage Display Peptide and Insulin Receptor

Luminescent quantum dots(QDs) are a promising alternative to organic dyes for fluorescence-based applications. Filamentous phage was labeled with QDs and its infective capability is observed after labeling. The experimental result shows that the labeling phage can still infect E.coli K91 normally. We have also developed the procedures for using QDs to label CHO-IR cells and Mouse Kidney tissue slice. The tissue slice remained stably labeled for over 10 d and the cells remained stably labeled even for over 5 months.