| 同位素内标-液相色谱-串联质谱法测定动物源性食品中氯霉素残留 |
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| 引用本文： | 陈君义,孙慧宇,舒永兰,王云飞.同位素内标-液相色谱-串联质谱法测定动物源性食品中氯霉素残留[J].化学分析计量,2011,20(2):46-48. |
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| 作者姓名： | 陈君义 孙慧宇 舒永兰 王云飞 |
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| 作者单位： | 1. 张家港出入境检验检疫局,张家港,215600
2. 徐州出入境检验检疫局,徐州,221006 |
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| 摘 要： | 加入氯霉素同位素内标的动物源性样品(龙虾、肠衣)经乙酸乙酯提取浓缩、正己烷去脂后,采用液-液萃取,水相经0.45μm滤膜过滤后,用液相色谱-串联质谱定量.在0.1、0.2,0.3μg/kg3个浓度水平上进行添加回收试验.回收率为71.8%～90.0%,检出限为0.05μg/kg.该法简化了前处理操作步骤,缩短了检测时间...
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| 关 键 词： | 同位素内标 液相色谱-串联质谱 动物源性食品 氯霉素 |
DETERMINATION OF CHLORAMPHENICOL RESIDUE IN FOOD OF ANIMAL ORIGIN BY ISOTOPE INTERNAL STANDARD-LIQUID CHROMATOGRAPHY- TANDEM MASS SPECTROMETRY |
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| Chen Junyi,Sun Huiyu,Shu Yonglan,Wang Yunfei.DETERMINATION OF CHLORAMPHENICOL RESIDUE IN FOOD OF ANIMAL ORIGIN BY ISOTOPE INTERNAL STANDARD-LIQUID CHROMATOGRAPHY- TANDEM MASS SPECTROMETRY[J].Chemical Analysis And Meterage,2011,20(2):46-48. |
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| Authors: | Chen Junyi Sun Huiyu Shu Yonglan Wang Yunfei |
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| Institution: | 1. Zhangjiagang Entry -Exit Inspection ＆ Quarantine Bureau, Zhangjiagang 215600, China; 2. Xuzhou Entry - Exit Inspection ＆ Quarantine Bureau, Xuzhou 221006, China) |
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| Abstract: | Chloramphenicol residue in food of animal origin was extracted by ethyl acetate and defatted by hexane with isotope marked ehloramphenieol spiked. After liquid - liquid extraction, the aqueous phase was filtered through 0.45 μm film and analyzed by liquid chromatography - tandem mass spectrometry. The recovery experiments were conducted at levels of 0.1, 0.2, 0.3 μg/kg with recovery range of 71.8% -90.0%. The limit of quantification was 0.05 μg/kg. The method can provide precise results with simple pretreatment, short detection time and lower cost. |
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| Keywords: | isotope internal standard liquid chromatography - tandem mass spectrometry food of animal origin ehloramphenieol |
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