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Differentiation of neurons from neural precursors generated in floating spheres from embryonic stem cells
Authors:Huawei Li   Hong Liu   C Eduardo Corrales   Jessica R Risner   Jeff Forrester   Jeffrey R Holt   Stefan Heller  Albert SB Edge
Affiliation:(1) Department of Otolarygology, EENT Hospital of Shanghai Medical College of Fudan University, Institute of Biomedical Sciences, Fudan University Shanghai, 200031, PR China;(2) Departments of Otolaryngology-HNS and Molecular and Cellular Physiology, Stanford University School of Medicine, 94305 Stanford, CA, USA;(3) Department of Otology and Laryngology, Harvard Medical School, 02115 Boston, MA, USA;(4) Eaton-Peabody Laboratory, Massachusetts Eye and Ear Infirmary, 02114 Boston, MA, USA;(5) Program in Speech and Hearing Bioscience and Technology, Division of Health Science and Technology, Harvard and MIT, 02139 Cambridge, MA, USA;(6) Department of Neuroscience, University of Virginia, 22908 Charlottesville, VA, USA;(7) Department of Otolaryngology, University of Virginia, 22908 Charlottesville, VA, USA
Abstract:

Background  

Neural differentiation of embryonic stem (ES) cells is usually achieved by induction of ectoderm in embryoid bodies followed by the enrichment of neuronal progenitors using a variety of factors. Obtaining reproducible percentages of neural cells is difficult and the methods are time consuming.
Keywords:
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