UV-ablation nanochannels in micro/nanofluidics devices for biochemical analysis |
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Authors: | Wang Chen Ouyang Jun Gao Hong-Li Chen Heng-Wu Xu Jing-Juan Xia Xing-Hua Chen Hong-Yuan |
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Institution: | a Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, 22 Hankou Road, Nanjing, Jiangsu 210093, China b Institute of Microanalytical Systems, Department of Chemistry, Zhejiang University, Hangzhou 310058, China |
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Abstract: | This paper presents a simple and cost-effective UV-ablation technique for fabrication of size-tunable nanofluidics devices via photochemical decomposition reaction. UV-irradiation through a PET photomask results in continuous decomposition of poly(carbonate) (PC), forming nanochannel and carboxyl groups on the surface of the etched PC. This photochemical decomposition process occurs at molecular scale, therefore, the depth of nanochannels can be controlled at nanometer level. The etching rate is estimated to be ca. 0.015 nm s−1. To demonstrate the potential application of the present UV-ablation technique, a nanochannel was fabricated and integrated with microchannels to form a micro/nanofluidics chip for protein concentration. Using this device, about 103-105 fold protein concentration can be achieved within 10 min. The present approach offers a simple and practical solution to fabricate nanofluidics devices at low-cost, and the resulting device could provide ideal platforms for μTAS towards various applications in biology and chemistry. |
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Keywords: | UV-ablation Size-tunable Micro/nanofluidics Protein concentration Bioanalysis |
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