血红蛋白与NO分子间相互作用的电化学表征

近年来 ,蛋白质的电化学研究引起了人们广泛兴趣1～ 5] .利用自组装技术4 ] 或将蛋白质分子固定于双层磷脂膜、水凝胶、表面活性剂膜及 Al2 O3膜上6～ 9] 等可成为制备蛋白质膜的有效手段 .血红蛋白(Mr=6 50 0 0 )是生物体内一类重要的蛋白 ,由于对其结构已有较清楚的认识 10 ] ,因而常被选作探讨生物大分子的电化学行为的模型分子 . NO是一种内皮细胞松弛因子、神经递质和免疫系统的媒介体 ,与生物体内的许多生理过程、疾病的产生与治疗有密切关系 11～ 13] .因此研究蛋白与 NO之间的相互作用并寻求监测 NO的各种途径具有重要意义…

Electrochemical Characterization on the Interaction Between Hemoglobin and NO Molecule

Nanocrystalline TiO 2 particles film has been considered as an eminent matrix for the immobilization of biomolecules due to its high biocompatiability, high protein loading and special binding ability. The direct electron transfer process of hemoglobin was achieved at nanosized TiO 2 film electrode, in which a well defined redox peak of hemoglobin could be observed. However, this peak decreased with the presence of NO, indicating the electrochemical activity of hemoglobin was inhibited by the interation between hemoglobin and NO. While the further evidence revealed that such activity could be recovered after releasing NO from hemoglobin with electrochemical oxidation method.