Analysis of in vivo and in vitro ESR spectra of H-flavin,an intrinsic spin label of flavoproteins

ESR experiments with fully deuterated flavoprotein have made possible the first determination of g-tensors and coupling constants for the radical form of this protein. Rotational diffusion correlation times for several in vitro and in vivo flavoprotein systems have been calculated and the utility of the saturation behavior of ²H-flavin radicals is demonstrated.