Biochemical assays of immobilized oligonucleotides with mass spectrometry |
| |
Authors: | Tsubery Haim Mrksich Milan |
| |
Affiliation: | Department of Chemistry, Howard Hughes Medical Institute, The University of Chicago, Chicago, Illinois 60637, USA. |
| |
Abstract: | This paper reports the use of mass spectrometry to characterize oligonucleotides immobilized to the surfaces of biochips. Biotinylated oligonucleotides were immobilized to self-assembled monolayers that present a streptavidin layer and then treated with a complementary strand to present short duplexes. Treatment of the surface with 5-methoxysalicylic acid and ammonium citrate matrix allows the individual oligonucleotides to be observed by matrix-assisted laser desorption/iozation and time-of-flight mass spectrometry (MALDI-TOF MS). Examples are shown wherein this method is applied to assays of hybridization, of cleavage by a deoxyribozyme, of a dephosphorylation reaction, and of the adducts formed on treatment of DNA with cis-platin. This work provides an early example of the application of mass spectrometry to DNA biochips and may substantially expand the applications of the now common oligonucleotide arrays. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|