| A New Protocol for Solubilization, Refolding and Purification of Recombinant Human Granulocyte Colony-stimulating Factor in Inclusion Bodies |
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| 引用本文： | Jia Hua LIU Chao Zhan WANG Xin Du GENG. A New Protocol for Solubilization, Refolding and Purification of Recombinant Human Granulocyte Colony-stimulating Factor in Inclusion Bodies[J]. 中国化学快报, 2006, 17(6): 799-802 |
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| 作者姓名： | Jia Hua LIU Chao Zhan WANG Xin Du GENG |
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| 作者单位： | Jia Hua LIU，Chao Zhan WANG，Xin Du GENG Institute of Modern Separation Science，Key Laboratory of Separation Science in Shaanxi Province，Northwest University，Xi’an 710069 |
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| 摘 要： | Recombinant human granulocyte colony-stimulating factor (rhG-CSF) is a multifunctio- nal cytokine, which plays a key role in the immune system. High-level expression of rhG-CSF in Escherichia Coli (E.Coli) often accumulates in vivo as insoluble aggregates…
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| 关 键 词： | 重组人粒细胞群体刺激因子 碱性溶解 增溶作用 蛋白质摺叠 生物活性 |
| 收稿时间： | 2005-12-01 |
A New Protocol for Solubilization, Refolding and Purification of Recombinant Human Granulocyte Colony-stimulating Factor in Inclusion Bodies |
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| Jia Hua LIU,Chao Zhan WANG,Xin Du GENG. A New Protocol for Solubilization, Refolding and Purification of Recombinant Human Granulocyte Colony-stimulating Factor in Inclusion Bodies[J]. Chinese Chemical Letters, 2006, 17(6): 799-802 |
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| Authors: | Jia Hua LIU Chao Zhan WANG Xin Du GENG |
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| Abstract: | |
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| Keywords: | Recombinant human granulocyte colony-stimulating factor alkaline solution solubilization of inclusion bodies protein refolding. |
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