Affiliation: | a Institute of Pharmaceutical Chemistry, University of Szeged, Eötvös u 6, H-6720 Szeged, Hungary b Department of Pharmaceutical Chemistry, University of Medicine and Pharmacy, Gh. Marinescu Street 38, RO-4300 Targu Mures, Romania |
Abstract: | The applicability of (1R,2R)-1,3-diacetoxy-1-(4-nitrophenyl)-2-propyl isothiocyanate [(R,R)-DANI] as a recently developed chiral derivatizing agent for the enantioseparation of a series of β-blockers is described. The thiourea diastereomers formed were analyzed by reversed-phase high-performance liquid chromatography, mixtures of water and methanol or acetonitrile being used for elution. Conditions of derivatizations (temperature, reagent excess and reaction time) were optimized, and the effects of organic modifiers on the retention and separation were investigated; the diastereomers could readily be baseline separated with methanol-containing mobile phases with resolutions between 1.58 and 2.72. |