Enhanced Recovery and Purification of P(3HB-co-3HHx) from Recombinant Cupriavidus necator Using Alkaline Digestion Method |
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| Authors: | Siti Nor Syairah Anis M I Nurhezreen K Sudesh A A Amirul |
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| Institution: | 1. School of Biological Sciences, Universiti Sains Malaysia, 11800, Minden, Penang, Malaysia
2. Malaysian Institute of Pharmaceuticals and Nutraceuticals, MOSTI, Minden, Malaysia
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| Abstract: | A simple, efficient and economical method for the recovery of P(3HB-co-3HHx) was developed using various chemicals and parameters. The initial content of P(3HB-co-3HHx) in bacterial cells was 50?C60?wt%, whereas the monomer composition of 3HHx used in this experiments was 3?C5?mol%. It was found that sodium hydroxide (NaOH) was the most effective chemical for the recovery of biodegradable polymer. High polyhydroxyalkanoate purity and recovery yield both in the range of 80?C90?wt% were obtained when 10?C30?mg/ml of cells were incubated in NaOH at the concentration of 0.1?M for 60?C180?min at 30?°C and polished using 20?% (v/v) of ethanol. |
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