Determination of cisapride in human plasma by high-performance liquid chromatography with fluorescence detection |
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Authors: | B. Dalmadi Kiss K. Balogh Nemes I. Klebovich |
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Affiliation: | (1) Department of Pharmacokinetics, EGIS Pharmaceuticals Ltd, P.O. Box 100, 1475 Budapest, Hungary |
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Abstract: | Summary A new sensitive HPLC-FLD method has been developed and validated for the determination of cisapride in human plasma for a bioequivalence study. A gradient method was used to remove late-eluting plasma components of no interest. The separation was performed on a Li-ChroCART 250-4 Purospher RP-18 (5 μm particle) analytical column fitted with a LiChroCART 4-4 Purospher RP-18 endcapped (5 μm particle) guard column. The excitation and emission wavelengths were 295 and 350 nm during fluorescence detection. The calibration plot was linear in the range of 5–200 ng mL−1. A demethoxy analogue of cisapride was used as internal standard. |
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Keywords: | Column liquid chromatography Solid-phase extraction Cisapride Human plasma Bioequivalence study |
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