Submerged culture screening of two strains ofStreptomyces sp. with high keratinolytic activity |
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Authors: | Garcia-Kirchner O. Bautista-Ramirez Ma. E. Segura-Granados M. |
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Affiliation: | (1) Departamento de Bioprocesos, Unidad Profesional Interdisciplinaria de Biotechnologís (UPIBI/IPN) del Institute Politécnico Nacional, Av. Acueducto s/n Barrio La Laguna, 07340 Ticomán, Zacatenco, México, D.F. |
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Abstract: | Keratinases can be used for the production of potentially important hydrolyzed proteins and chemicals. This study investigated the keratinolytic activity ofStreptomyces sp on keratinaceous materials like wool. High levels of proteolytic and keratinolytic activity were obtained after 96 h of culture when two Streptomyces sp strains were grown on basal medium containing mineral salts and 3% (w/v) of defatted wool as a source of energy, carbon, and nitrogen. The cell-free culture filtrates exhibited rapid proteolytic digestion of keratin powder. Currently, the authors are testing whether the enzymatic activity obtained is in fact keratinolytic, and not only an alkaline protease activity. |
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Keywords: | KeywordHeading" >Index Entries Keratinases enzymatic hydrolysis Streptomyces sp M-Zyme alkaline proteases |
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