| 番红花红O-肝素钠体系的光谱分析及应用 |
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| 引用本文： | 郭小群,马迪.番红花红O-肝素钠体系的光谱分析及应用[J].四川理工学院学报(自然科学版),2007,20(6):91-94. |
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| 作者姓名： | 郭小群 马迪 |
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| 作者单位： | 1. 西南大学化学化工学院,重庆,400715;四川理工学院化学系,四川,自贡,643000
2. 四川理工学院化学系,四川,自贡,643000 |
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| 摘 要： | 在pH6.0的Britton2Robinson(B-R)缓冲溶液中,番红花红O与肝素钠相互作用,形成缔合物而导致溶液吸收光谱发生变化,并对光谱变化进行了对比研究,体系的共振瑞利散射光谱带落在吸收光谱带上,并且散射峰与吸收峰对应。体系的最大共振散射峰在334nm处,而在335nm对应的吸收最弱。这表明,当大分子聚合物吸收了特定波长的能量时,它的外层电子跃迁到更高能态。并且研究了共存物质的影响,表明此方法选择性好,用于肝素钠注射液效价的测定,RSD小于1%。
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| 关 键 词： | 番红花红O 肝素钠 分光光度 RRS |
| 文章编号： | 1673-1549（2007）06-0091-04 |
| 收稿时间： | 2007-05-21 |
| 修稿时间： | 2007年5月21日 |
Analysis and Application for the Spectra of Safranine O- Heparin Sodium System |
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| GUO Xiao-qun,MA Di.Analysis and Application for the Spectra of Safranine O- Heparin Sodium System[J].Journal of Sichuan University of Science & Engineering:Natural Science Editton,2007,20(6):91-94. |
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| Authors: | GUO Xiao-qun MA Di |
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| Abstract: | The interaction of safranine o and heparin sodium was studied by the comparison to the variation of absorption spectra in B-R buffer solution with pH=6.0.The position to the strongest resonance rayleigh scattering(RRS)peak(334nm)was corresponding to that of the weakest absorption(335nm).It indicates that the out-shell electrons of macromolecule will transition to a higher energy level when the absorption of hv with specified wavelength is taken place.Furthermore,thestudy for the influence of coexistent substances has shown a satisfied seleCtivity and result which was achieved in the determination of the potencyof heparin sodium injection with RSD<1%. |
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| Keywords: | RRS |
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