| 1,3-环己二酮荧光法测定食品中的痕量甲醛 |
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| 引用本文： | 张建霞,郭惠,刘希东,李原芳.1,3-环己二酮荧光法测定食品中的痕量甲醛[J].渝西学院学报(自然科学版),2011(4):50-53. |
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| 作者姓名： | 张建霞 郭惠 刘希东 李原芳 |
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| 作者单位： | [1]重庆文理学院化学与环境工程学院,重庆永川402160 [2]西南大学化学化工学院重庆市现代分析化学重点实验室,重庆北碚400715 |
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| 基金项目： | 重庆市教委科学技术研究项目（KJ071209）; 重庆市现代分析化学重点实验室开放基金计划资助项目（201001） |
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| 摘 要： | 甲醛与1,3-环己二酮-组胺在弱酸性缓冲介质中反应生成强荧光化合物,由此建立一种测定食品中痕量甲醛的荧光分光光度法.在优化的实验条件下,反应体系的相对荧光强度与甲醛浓度呈良好的线性关系,该方法的线性范围为0.02～1.00μg/mL,检出限为1.8×10-3μg/mL,对0.75μg/mL甲醛平行测定11次,其相对标准偏差为2.0%.该方法用于食品中甲醛的测定,回收率为81.2%～87.5%,与乙酰丙酮法对照,结果基本一致.
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| 关 键 词： | 甲醛 1 3-环己二酮 组胺 荧光光度法 |
Spectrofluorometric determination of trace amounts of formaldehyde in food after derivatization with 1,3-Cyclohexanedione |
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| Authors: | ZHANG Jian-xia GUO Hui LIU Xi-dong LI Yuan-fang |
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| Institution: | 1.College of Chemistry and Environment Engineering,Chongqing University of Arts and Sciences,Yongchuan Chongqing 402160,China;2.Chongqing Key Laboratory of Analysis Chemistry,College of Chemistry and Chemiscal Engineering,Southwest University,Beibei Chongqing 400715,China) |
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| Abstract: | It is found that formaldehyde can react with 1,3-Cyclohexanedione-histamine in the subacidity medium and produce hyperfluorescence componds.Under the optimum conditions,its relative fluorescence intensity is linearly related to the concentration of formaldehyde in the range of 0.02～1.00 μg/mL with a detection limit of 1.8×10-3 μg/mL,the relative standard deviation for the determination of 0.75 μg/mL formaldehyde was 2.0%.The method was applied to determination of formaldehyde in food and the recovery is in the range of 81.2%～87.5%.Compared with acetyacetone method,the results were well. |
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| Keywords: | formaldehyde 1 3-cyclohexanedione histamine spectrofluorometric |
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