Molecular diversity of rat brain proteins as revealed by proteomic analysis |
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Authors: | Jae-Won?Yang Jean-Fran?ois?Juranville Harald?H?ger Michael?Fountoulakis Email author" target="_blank">Gert?LubecEmail author |
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Institution: | (1) Department of Pediatrics, University of Vienna, Vienna, Austria;(2) F. Hoffmann-La Roche, Genomics and Proteomics Technologies, Basel, Switzerland;(3) Institute for Animal Genetics, Himberg, Austria;(4) Center of Basic Research, Foundation for Biomedical Research of the Academy of Athens, Athens, Greece |
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Abstract: | Summary Multiple protein expression forms (MPEFs) presenting splicing forms or co- and posttranslation modifications, account for
the vast diversity, the myriad of gene products and clearly indicate problems which proteomics research is facing. In the
present study, we generated a rat brain map representing MPEFs by the use of an analytical method based on two-dimensional
electrophoresis combined with mass spectrometry. Forty-nine individual proteins were selected that showed more than two spots,
resulting altogether into a total number of 357 expression forms. Some proteins showed large MPEFs numbers as e.g. tubulin
alpha-1 chain (24 spots), ATP synthase alpha-chain (28), beta chain (17) or septin 7 (13).
The molecular diversity observed in this map clearly shows that immunochemical or even protein chemical results from expressional
studies have to be interpreted with caution, in particular if one dimensional electrophoretic or western blot techniques are
applied and MPEFs are poorly resolved. |
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Keywords: | mass spectrometry protein expression form protein modification proteomics rat brain two-dimensional gel electrophoresis |
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