Pyrenebutanoate as a dynamic protein modifier for fluorometric detection in capillary zone electrophoresis |
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| Authors: | Horká Marie Slais Karel |
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| Affiliation: | Institute of Analytical Chemistry, Academy of Sciences of the Czech Republic, Brno, Czech Republic. horka@iach.cz |
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| Abstract: | Pyrenebutanoate as the amphiphilic fluorescent compound is suggested as a buffer additive in capillary zone electrophoresis (CZE) for a dynamic modification of several protein samples. Using deuterium lamp UV excitation for the on-column fluorometric detection, minimum detectable amounts in the amol-range of the proteins sampled on the CZE capillary was achieved. |
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