A Probe Design for the Acquisition of Homonuclear, Heteronuclear, and Inverse Detected NMR Spectra from Multiple Samples

A new probe design is presented for obtaining homonuclear, heteronuclear, and inverse detected NMR spectra from more than one sample in the same total data acquisition time as for a single sample, thus increasing data acquisition efficiency. Specifically, a two-coil system, with each solenoidal coil impedance matched to 50 Omega at both proton and nitrogen frequencies, has been designed for operation at 11.7 T with an observe volume of 15 microL for each coil. Isolation between the two frequencies for each individual coil, and at each frequency between coils, was greater than 30 dB. Two-dimensional COSY and HMQC spectra were obtained with negligible NMR cross-talk between the two coils.