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一种确定转瓶中昆虫细胞摄氧率的简便方法
引用本文:赵佼,谭文松.一种确定转瓶中昆虫细胞摄氧率的简便方法[J].华东理工大学学报(自然科学版),1997,23(5):540-544.
作者姓名:赵佼  谭文松
作者单位:华东理工大学生物反应器工程国家重点实验室
摘    要:提出一种确定转瓶中昆虫细胞摄氧率(OUR)的简便方法。不同溶氧水平的转瓶实验表明:在考虑了小型培养装置中溶氧解吸过程的这种方法不仅能正确反映细胞OUR的变化状况,而且能借助OUR迅速判断培养细胞所处生理状态。

关 键 词:昆虫细胞  解吸  生理状态  摄氧率  转瓶

A Simple Method for OUR Determination of the Insect Cells Cultured in Spinner Flasks
Zhao Jiao ,Tan Wensong,Zhou Yajing,Zhang Honghua and Yu Juntang.A Simple Method for OUR Determination of the Insect Cells Cultured in Spinner Flasks[J].Journal of East China University of Science and Technology,1997,23(5):540-544.
Authors:Zhao Jiao  Tan Wensong  Zhou Yajing  Zhang Honghua and Yu Juntang
Institution:Zhao Jiao *,Tan Wensong,Zhou Yajing,Zhang Honghua and Yu Juntang
Abstract:A simple and convenient method was developed for OUR determination of the insect cells cultured in spinner flasks.The experiments for spinner flasks have shown that the method which takes into account the oxygen desorption process could not only accurately reflect the OUR trend during the cultivation but also promptly determine the physiological state of cultured cells according to OUR values.
Keywords:insect cell  desorption  physiological state  OUR  spinner flask
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