Analysis of chloramphenicol residues in swine tissues and milk: comparative study using different screening and quantitative methods |
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Authors: | C van de Water N Haagsma |
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Institution: | Faculty of Veterinary Medicine, Department of the Science of Food and Animal Origin, University of Utrecht, The Netherlands. |
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Abstract: | Both screening and quantitative methods for chloramphenicol residues in swine tissues and milk were compared, using samples from animals treated with chloramphenicol. For screening purposes a previously developed streptavidin-biotin enzyme-linked immunosorbent assay and a commercially available immunochemical card test were used. For quantitative purposes two previously developed high-performance liquid chromatographic procedures were applied using antibody-mediated clean-up and solid-phase extraction. Some improvements in both methods were also described. The results obtained with the screening tests and those obtained with the quantitative methods correspond well with each other. Using a combination of these methods, an effective control of residues of chloramphenicol can be performed in milk from the 1 microgram/kg level and in swine tissues from the 10 micrograms/kg level. |
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