Structure and properties of porous composite membranes of regenerated silk fibroin and poly(vinyl alcohol) and biosensing of glucose via Meldola blue dispersed in polyester ionomer as an electron transfer mediator |
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| Authors: | H Liu Huihong Deng Kang Sun Deyao Qi Jiaqi Deng Yongcheng Liu Tongyin Yu |
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| Institution: | (1) Department of Chemistry and Chemical Engineering, Shanghai University, Shanghai 200 072, P. R. China, CN;(2) Department of Chemistry, Fudan University, Shanghai 200 433, P. R. China, CN;(3) Department of Macromolecular Science, Fudan University, Shanghai 200 433, P. R. China, CN |
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| Abstract: | Porous composite membranes of regenerated silk fibroin and poly(vinyl alcohol) were prepared by adding polyethyleneglycol
to the composite solution to reduce the mass-transfer resistance to the diffusion of substrate material transport; their surfaces
were visualized with scanning electron microscopy. An amperometric glucose biosensor employing Meldola blue dispersed in polyester
ionomer as electron transfer mediator was prepared to test the feasibility and workability of the composite membrane as immobilization
matrix for glucose oxidase. The cationic exchange property of the polyester ionomer was employed to provide high local concentrations
of Meldola blue (MB+) in the polymer film via ion exchange. Performance and characteristics of the glucose biosensor were evaluated with respect
to response time, detection limit, applied potential, thickness of polyester ionomer membrane, pH and temperature. The glucose
biosensor possesses a variety of advantages including easy maintenance of enzyme, simplicity of construction, fast response
time and high stability.
Received: 13 May 1996 / Revised: 30 July 1996 / Accepted: 2 August 1996 |
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