不同微生物组合对石油烷烃降解性能的比较分析

以大连石油污染海域的海洋沉积物作为分离样品,以天然海水培养基分离获得13种海洋石油降解微生物,并通过模拟海底低温微氧环境,以原油作为唯一碳源,评价了13种微生物的石油降解性能。GC-MS分析结果表明,随着降解时间的增加,组合G6及其构建菌株P73和P83对烷烃的降解率逐渐升高。对于C>32的长链烷烃部分,尤其对于石油中的难降解组分如姥鲛烷(Pr)和植烷(Ph)的降解,组合G6的降解效果明显优于单菌株。由高效石油降解菌菌株P73和菌株P83构建的组合G6对于石油烷烃中很难降解和风化的长链烷烃具有强大的协同降解作用。

Analysis for Degradation Ability of the Mixed Bacterial Consortium to Alkanes

The marine sediment samples were collected from polluted zone in Dalian, and 13 species of marine petroleum degrading microorganisms were isolated from these marine sediment samples with the seawater medium. While the crude oil was used as the sole carbon source, the petroleum degradation abilities of 13 species of bacteria were evaluated by simulating the low temperature and micro-oxygen environment of the seabed. The results of GC-MS analysis showed that the degradation rate of alkanes by combination G6 and its constructed strains P73 and P83 increased gradually with the increase of degradation time. But for high alkane part of C>32, especially for refractory components such as Pristane (Pr) and Phytane (Ph), the degradation ability of the consortium G6 was significantly higher than that of the single strain. The consortium G6 consisting of strains P73 and P83 showed a strong synergistic degradation ability for paraffin components.