Interaction of dinucleotides with divalent metal ions: A circular dichroism study |
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| Affiliation: | 1. Department of Environmental Science, Zhejiang University, Hangzhou, Zhejiang 310058, China;2. Zhejiang Provincial Key Laboratory of Organic Pollution Process and Control, Hangzhou, Zhejiang 310058, China;1. Centre for Advanced Studies in Botany, University of Madras, Guindy Campus, Chennai, Tamil Nadu, Pin Code – 600025, India;2. Department of Botany, Bharathidasan University, Tiruchrappalli, Pin Code – 620024, India;3. Environment Information System (ENVIS-Centre), Department of Zoology, University of Madras, Guindy Campus, Chennai, Tamil Nadu, Pin Code – 600025, India;1. Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan;2. RIKEN SPring-8 Center, 1-1-1 Kouto, Sayo, Hyogo 679-5148, Japan;1. Department of Chemistry, Center for Innovative Technology, Vanderbilt Institute for Integrative Biosystems Research and Education, and Vanderbilt Institute of Chemical Biology, Vanderbilt University, Nashville, TN, 37235, United States;2. Institute of Biochemistry, Faculty of Biosciences, Pharmacy and Psychology, Leipzig University, 04103 Leipzig, Germany;3. Department of Molecular Systems Biology, Helmholtz-Centre for Environmental Research – UFZ, 04318 Leipzig, Germany |
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| Abstract: | The interactions between NAD+ and NADP+ and the divalent ions Mg2+, Mn2+ and Co2+ have been studied by circular dichroism in the UV range. A visible C.D. study has been carried on the Co2+ complexes.The observed changes of the UV and C.D. as a function of temperature clearly show that the metal ion does not bridge the purine base and the nicotinamide.The observed changes of the UV C.D. as a function of temperature clearly show that the metal ion does not bridge the purine base and the nicotinamide.The strong modification of the UV C.D. and the appearance of visible C.D. in 0.1 M NAD+ or NADP+ solutions in presence of Co2+ which occurs when the N(1) adenine atom is deprotonated, has been ascribed to the formation of a 1:2 Co2+-dinucleotide complex where the two adenine bases are stacked. |
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