Spectrophotometric studies of kanamycin-copper complex and determination of kanamycin sulfate using an auxiliary ligand |
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| Affiliation: | 1. Department of Pharmacology, Faculty of Veterinary Medicine, University of Murcia, Campus de Espinardo, 30.100 Murcia, Spain;2. Escuela de Medicina Veterinaria, Colegio de Ciencias de la Salud, Universidad San Francisco de Quito, EC 170157 Cumbayá, Ecuador;1. Department of Neuroscience, Vickie and Jack Farber Institute for Neuroscience, Sidney Kimmel Medical College at Thomas Jefferson University, 233 S. 10th St., Bluemle Life Sciences Building - Room 245, Philadelphia, PA 19107, United States of America;2. School of Biomedical Engineering, Science and Health Systems, Drexel University, 3141 Chestnut Street, Bossone 7-716, Philadelphia, PA 19104, United States of America;3. Department of Biology, Arcadia University, 450 S Easton Rd, 220 Boyer Hall, Glenside, PA 19038, United States of America |
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| Abstract: | Spectrophotometric investigation of the deep blue, water soluble complex of kanamycin sulphate with bivalent copper in the alkaline medium has been carried out using sodium potassium tartrate as an auxiliary ligand. The complex is found to absorb maximum at 580 nm and pH 8.0. Job's method and mole ratio method have shown the composition of the complex to be 1:2. Conditional stability constant has been determined by Mukherji and Dey method and Raghav Rao's method and the log Ks value by these two methods is found to be 6.69 and 7.59 respectively. The colour of the complex obeys Beer's law within the range 0.3 – 3.0 mg of kanamycin sulphate/ml of the solution. The technique is found to be suitable for the quantitative estimation of kanamycin sulphate. |
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