Fabrication of DNA polymer brush arrays by destructive micropatterning and rolling-circle amplification |
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Authors: | Barbee Kristopher D Chandrangsu Matt Huang Xiaohua |
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Institution: | Department of Bioengineering, University of California San Diego, La Jolla, CA 92093-0412, USA. |
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Abstract: | A method for fabricating DNA polymer brush arrays using photolithography and plasma etching followed by solid-phase enzymatic DNA amplification is reported. After attaching oligonucleotide primers to the surface of a glass coverslip, a thin layer of photoresist is spin-coated on the glass and patterned via photolithography to generate an array of posts in the resist. An oxygen-based plasma is then used to destroy the exposed oligonucleotide primers. The glass coverslip with the primer array is assembled into a microfluidic chip and DNA polymer brushes are synthesized on the oligonucleotide array by rolling-circle DNA amplification. We have demonstrated that the linear polymers can be rapidly synthesized in situ with a high degree of control over their density and length. |
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Keywords: | DNA polymer brush arrays functional coating microfluidics photolithography solid‐phase rolling circle amplification |
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