Hydrogen Evolution from L-Lactate and L-Malate with the Combination of Lactate Dehydrogenase or Malate Dehydrogenase and Hydrogenase from A. Eutrophus H16

Hydrogen evolution system from L-lactate and L-malate consisting of lactate dehydrogenase or malate dehydrogenase and hydrogenase from cell free extracts of Alcaligenes eutrophus H16 was established. When the solution containing L-lactate, lactate dehydrogenase, NAD and hydrogenase was incubated at 30°C hydrogen evolution was observed. Similarly, the hydrogen evolution was also observed from the L-malate incubated at 30°C.