Enhanced OH radical generation by dual-frequency ultrasound with TiO2 nanoparticles: Its application to targeted sonodynamic therapy |
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| Institution: | 1. Institute of Nature and Environmental Technology, Kanazawa University, Kanazawa 920-1192, Japan;2. Division of Material Engineering, Graduate School of Natural Science and Technology, Kanazawa University, Kanazawa 920-1192, Japan;3. Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, Kobe 657-8501, Japan;4. Department of Bioengineering Sciences, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan |
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| Abstract: | The present study demonstrated the enhanced hydroxyl (OH) radical generation by combined use of dual-frequency (0.5 MHz and 1 MHz) ultrasound (US) and titanium dioxide (TiO2) nanoparticles (NPs) as sonocatalyst. The OH radical generation became the maximum, when 0.5 MHz US was irradiated at an intensity of 0.8 W/cm2 and 1 MHz US was irradiated at intensities at 0.4 W/cm2 in the presence of TiO2 NPs under the examined conditions. After incorporation of TiO2 NPs modified with targeting protein pre-S1/S2, HepG2 cancer cells were subjected to the dual-frequency US at optimum irradiation intensities (“targeted-TiO2/dual-US treatment”). Growth of the HepG2 cells was reduced by 46% compared with the control condition after irradiation of dual-frequency US for 60 s with TiO2 NPs incorporation. In contrast, HepG2 cell growth was almost the same as that in the control condition when cells were irradiated with either 0.5 MHz or 1 MHz ultrasound alone without TiO2 NP incorporation. |
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| Keywords: | Dual-frequency Ultrasound Titanium dioxide Nanoparticles Hydroxyl radical Sonodynamic therapy |
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