Novel design of multicapillary arrays for high-throughput DNA sequencing |
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Authors: | Tsupryk Andriy Gorbovitski Michael Kabotyanski Evgeni A Gorfinkel Vera |
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Affiliation: | Electrical and Computer Engineering Department, State University of New York at Stony Brook, Stony Brook, NY, USA. atsupryk@ic.sunysb.edu |
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Abstract: | A novel approach to design and optimize linear multicapillary arrays (LMCAs) for high-throughput DNA sequencing is proposed. A significant increase in the number of capillary lanes is obtained due to the use of composite insertions alternately placed between working capillaries of the array and a specific combination of refractive indices of the DNA separation matrix, capillary glass, the insertions and a medium which surrounds the capillary array. Theoretical and experimental studies showed that in conjunction with a dual-side laser illumination scheme, the proposed LMCA design allows a simultaneous uniform irradiation of as many as 550 working capillaries. |
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Keywords: | Capillary array Capillary electrophoresis DNA sequencing Fluorescence detection Laser illumination |
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