| 超高效液相色谱-串联质谱法测定鸡肉中的4种聚醚类抗球虫药物残留 |
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| 引用本文： | 毕言锋,徐倩,王树槐,汪霞.超高效液相色谱-串联质谱法测定鸡肉中的4种聚醚类抗球虫药物残留[J].分析试验室,2009,28(7). |
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| 作者姓名： | 毕言锋 徐倩 王树槐 汪霞 |
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| 作者单位： | 中国兽医药品监察所,国家兽药残留基准实验室,北京,100081 |
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| 摘 要： | 采用乙腈直接提取、石墨化碳黑固相萃取小柱净化、UPLC快速分离,串联质谱检测、成功建立了鸡肉中莫能菌素、马杜菌素、盐霉素、拉沙洛西等4种抗球虫药物的多残留检测方法.对样品提取、净化过程和仪器条件进行了优化改进,经Waters C18 columm分离、正离子电喷雾电离模式测定,4种药物的检测限为0.2μg/kg,最低定量限为0.5μg/kg.选取0.5、1.0、2.0μg/kg 3个质量分数进行空白添加回收率实验,回收率为71.9%～100.9%.批内RSD 2.7%～15%,批间RsD为1.7%～10%.各项技术指标满足国内外法规的要求,适合大量样品中聚醚类抗球虫药物残留的确认检测.
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| 关 键 词： | 超高效液相色谱-串联四极杆质谱 聚醚类抗球虫药物 鸡肉 |
Simultaneous determination of four polyethers coccidiostats in chicken muscles using ultra perfonnace liquid chromatography tandem mass |
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| BI Yan-feng,XU Qian,WANG Shu-huai,WANG Xia.Simultaneous determination of four polyethers coccidiostats in chicken muscles using ultra perfonnace liquid chromatography tandem mass[J].Chinese Journal of Analysis Laboratory,2009,28(7). |
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| Authors: | BI Yan-feng XU Qian WANG Shu-huai WANG Xia |
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| Institution: | National Reference Laboratories of Veterinary Drug Residue;China Institute of Veterinary Drug Control;Beijing 100081 |
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| Abstract: | A method for the simultaneous analysis of four polyethers coccidiostats in chicken muscles was developed by ultra performance liquid chromatography-tandem mass spectrometry.The residues from chicken muscles were extracted with acetonitrile,and the extracting solutions were purified using supelclean ENVI-Carb SPE cartridges.The four residues were separated on a Waters ACQUITY UPLCTM BEH C18 column,and detected by MS/MS with positive electrospray ionization mode.The limits of detection(LOD) for the four drugs... |
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| Keywords: | Ultra performance liquid chromatography-tandem mass spectrometry Polyethers coccidiostats Chicken muscles |
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