毛细管电泳法测定复方制剂中的硝酸咪康唑

毛细管电泳;高频电导检测;非接触式电导检测;硝酸咪康唑;复方制剂

Determination of Miconazole Nitrate in Medicines by Capillary Electrophoresis

A method for the determination of miconazole nitrate in two different medicines by capillary electrophoresis with high frequency conductivity detection(contactless conductivity detection) was(established).The electrophoretic parameters, such as the variety and concentration of buffer solution,separation voltage etc,were researched.Miconazole nitrate was separated and detected in a buffer(3.0 mmol/L lactic acid 10%ethanol(pH=4.0)) at 20.0 kV constant voltage.The linear dependence of the concentrations of miconazole nitrate ranged from 20.0 to 900 mg/L(r=0.999 4),and the limit of detection(LOD) reached 1.0 mg/L((S/N=)3).The RSDs for both intra-day and inter-day were less than 3% and the recoveries were 94.9%~101% and 95.3%~99.1% for Miconazole-nitrate-cream and Miconazole-nitrate-vaginal-suppositories(suppository)(respectively) at various spiked levels.This method may be useful for the determination of(miconazole) nitrate in compound preparations.It could be used for quality control of products due to its(simplicity),rapidity and sensitivity.