Rapid determination of glyphosate, glufosinate, bialaphos, and their major metabolites in serum by liquid chromatography-tandem mass spectrometry using hydrophilic interaction chromatography |
| |
Authors: | Yoshioka Naoki Asano Migiwa Kuse Azumi Mitsuhashi Takao Nagasaki Yasushi Ueno Yasuhiro |
| |
Affiliation: | Hyogo Prefectural Institute of Public Health and Consumer Sciences, 2-1-29, Arata-cho, Hyogo-ku, Kobe 652-0032, Japan. Naoki Yoshioka@pref.hyogo.lg.jp |
| |
Abstract: | We developed a simple and rapid method for the simultaneous determination of phosphorus-containing amino acid herbicides (glyphosate, glufosinate, bialaphos) and their major metabolites, aminomethylphosphonic acid (AMPA) and 3-methylphosphinicopropionic acid (MPPA), in human serum. Serum samples were filtrated through an ultrafiltration membrane to remove proteins. The filtrate was then washed with chloroform, and injected into a liquid chromatography-tandem mass spectrometry (LC-MS/MS) system. Chromatographic separation was achieved on a hydrophilic interaction chromatography (HILIC) column. Determination of the target herbicides and metabolites was successfully carried out without derivatization or solid phase extraction (SPE) cartridge clean-up. The recoveries of these compounds, added to human serum at 0.2μg/mL, ranged from 94% to 108%, and the relative standard deviations (RSDs) were within 5.9%. The limits of detection (LODs) were 0.01μg/mL for MPPA, 0.02μg/mL for AMPA, 0.03μg/mL for both glyphosate and glufosinate, and 0.07μg/mL for bialaphos, respectively. |
| |
Keywords: | Glyphosate Glufosinate Bialaphos Serum HILIC LC–MS/MS |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|