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Light Regulation of Protein Dimerization and Kinase Activity in Living Cells Using Photocaged Rapamycin and Engineered FKBP |
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| Authors: | Karginov Andrei V Zou Yan Shirvanyants David Kota Pradeep Dokholyan Nikolay V Young Douglas D Hahn Klaus M Deiters Alexander |
| Institution: | Department of Pharmacology and Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, United States, and Department of Chemistry, North Carolina State University, Raleigh, North Carolina 27695, United States. |
| Abstract: | We developed a new system for light-induced protein dimerization in living cells using a photocaged analogue of rapamycin together with an engineered rapamycin binding domain. Using focal adhesion kinase as a target, we demonstrated successful light-mediated regulation of protein interaction and localization in living cells. Modification of this approach enabled light-triggered activation of a protein kinase and initiation of kinase-induced phenotypic changes in vivo. |
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