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Cross-talk between BubR1 expression and the commitment to differentiate in adipose-derived mesenchymal stem cells |
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| Authors: | Janet Lee Chang Geun Lee Kyo-Won Lee and Chang-Woo Lee |
| Institution: | 1Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine, Suwon 440-746, Korea.;2Center for Molecular Medicine, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon 440-746, Korea.;3Department of Obstetrics and Gynecology, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine, Seoul 110-746, Korea. |
| Abstract: | BubR1 mitotic checkpoint kinase monitors attachment of microtubules to kinetochores and links regulation of the chromosome-spindle attachment to mitotic checkpoint signaling. Defects in BubR1-mediated signaling severely perturb checkpoint control and are linked to diseases such as cancer. Studies using BubR1 mouse models suggest that BubR1 activities prevent premature aging and infertility. In this study, we show that BubR1 depletion in human adipose-derived mesenchymal stem cells (ASCs) precedes loss of the differentiation potential and induction of replicative senescence. These effects occur independently of p16INK4A expression and may involve DNA methylation. Our results reveal a new and unsuspected feature of BubR1 expression in regulation of adult stem cell differentiation. |
| Keywords: | adult stem cell BubR1 protein cell aging cell differentiation DNA methylation |