Purification of paeoniflorin from Paeonia lactiflora Pall. by high-speed counter-current chromatography |
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Authors: | Chen Feng Lu Hai-Tao Jiang Yue |
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Institution: | Department of Botany, The University of Hong Kong, Pokfulam Road, Hong Kong, China. sfchen@hkusua.hku.hk |
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Abstract: | High-speed counter-current chromatography was successfully used for the first time for the preparative separation and purification of paeoniflorin from the Chinese medicinal plant Paeonia lactiflora Pall. using a two-phase solvent system composed of n-butanol-ethyl acetate-water (1:4:5, v/v) in a single run. From 160 mg of the crude sample containing 22.0% paeoniflorin, 33.2 mg of paeoniflorin was yielded at 98.2% purity as determined by HPLC analysis. The recovery of paeoniflorin was 94.3%. |
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