His6-OPH enzyme-based bio-hybrid material for organophosphate detection |
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Authors: | Fran?i? Nina Ko?ak Aljo?a Lyagin Ilya Efremenko Elena N Lobnik Aleksandra |
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Institution: | (1) Faculty of Mechanical Engineering, Centre of Sensor Technology, University of Maribor, Smetanova 17, 2000 Maribor, Slovenia;(2) Faculty of Chemistry, Lomonosov Moscow State University, Moscow, 119992, Russia; |
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Abstract: | In this work, we report on the development of a bio-sensing film for the detection of organophosphorous compounds using sol–gel
technology. A novel sol–gel immobilization method employing tetraethoxysilane/3-glycidoxypropyltrimethoxysilane/water hybrid
material was developed and used to immobilize the hexahistidine-tagged organophosphorous hydrolase enzyme (His6-OPH). Bio-sensing layers with encapsulated His6-OPH of various structures (water/silane, precursor ratios) have been prepared. The optimal (P = 5:1, R = 188) bio-sensing layers retained 90% of the initial enzyme activity. Furthermore, the bio-sensing layer prepared by this
method was able to maintain its activity at or above 80% of its initial activity for 2 weeks. The bio-hybrid film also showed
excellent reusability and improved activity at neutral pH in comparison to the same enzyme in solution. |
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