Dynamic changes of nucleolar DNA configuration and distribution during the cell cycle in Allium sativum cells |
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| Authors: | Guangbin Shang Fengcai Wang Shui Hao Mingda Jiao |
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| Institution: | 1. Department of Medicine, Huzhou University, Huzhou 313000, China;2. Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;1. Parasitology Laboratory, Theodor Bilharz Research Institute, Giza, Egypt;2. Clinical Parasitology Department, Research Institute of Ophthalmology, Giza, Egypt;3. Ph.D. of Immunology and Parasitology, Egypt;4. Parasitology Department, Faculty of Medicine, Zagazig University, Egypt;5. Natural Sciences, Math and Science Program, University College, Abu Dhabi University, United Arab Emirates;6. Biological Sciences Department, King Faisal University, Saudi Arabia;7. Hydrobiology Department, National Research Centre, Dokki, Giza, Egypt;8. Biology Dept., Girls Science College, University of Dammam, Saudi Arabia;1. Jiu Jiang University, Jiangxi Province, 332000, China;2. Key Laboratory of Modern Preparation of Traditional Chinese Medicine, Ministry of Education, Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi, 330004, China;3. Institute of Genetics and Cytology, Northeast Normal University, Changchun, Jilin, 130024, China;1. Department of Biotechnology, Jamia Millia Islamia, Jamia Nagar, New Delhi 110025, India;2. Centre for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, Jamia Nagar, New Delhi 110025, India |
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| Abstract: | To investigate the correlation between subnucleolar structure and function, the precise distribution and configuration of nucleolar DNA during the cell cycle of Allium sativum were determined using the NAMA-Ur DNA-specific staining technique. We showed that nucleolar DNA is present in two forms: compacted chromatin clumps and a decondensed DNA cloud. The form of the DNA within the nucleolus varied greatly as the cell cycle progressed. During telophase, chromosomes extended into the prenucleolar body. In early G1 phase, DNA was only located in the fibrillar centers in the form of the condensed chromatin clump, while in mid-G1, S and G2 phases, the two forms of DNA were distributed in the fibrillar centers (FC) and dense fibrillar component (DFC). In prophase of mitosis, nucleolar DNA, along with FC and DFC, was linked into a network structure and condensed into a large chromatin clump. The area of the DNA cloud in the dense fibrillar component changed during different phases of the cell cycle. Our results demonstrated that the configuration of nucleolar DNA undergoes a series of decondensations and condensations during the cell cycle to fulfill the function of the nucleoli during the different phases. |
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