Design and Fabrication of Artificial Skin: Chitosan and Gelatin Immobilization on Silicone by Poly Acrylic Acid Graft Using a Plasma Surface Modification Method |
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| Authors: | Amir Salati Hamid Keshvari Akbar Karkhaneh Shahrouz Taranejoo |
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| Institution: | 1. Faculty of Biomedical Engineering , Amirkabir University of Technology , Tehran, Iran;2. Faculty of Biomedical Engineering , Islamic Azad University, Science and Research Branch , Tehran, Iran;3. School of Chemical Engineering , University of Tehran , Tehran, Iran |
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| Abstract: | Acrylic acid (AAc) was grafted onto the surfaces of polydimethylsiloxane (PDMS) films using a two-step oxygen plasma treatment. The first step of this method included oxygen plasma pretreatment of the PDMS films, immersion in AAc, and drying. The second step was carried out by plasma polymerization of the preadsorbed reactive AAc on the surfaces of the dried pretreated films. Then chitosan and gelatin were immobilized onto the poly(acrylic acid) grafted silicone through covalent bonding. The films were characterized by attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy and water contact angle measurements. Fibroblast cells (L929) were cultured onto the chitosan- and gelatin-immobilized poly(acrylic acid)-grafted silicone and poly(acrylic acid)-grafted silicone films. It was observed that the chitosan- and gelatin-immobilized surfaces showed significant cell growth in comparison with poly(acrylic acid)-grafted silicone samples. It seems that chitosan- and gelatin-immobilized surfaces may have an excellent potential to be used as a derm-like matrix. |
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| Keywords: | acrylic acid artificial skin graft polymerization plasma silicone surface modification |
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